3D printing technologies have been widely used in Tissue Engineering. The pneumatic dispensing system is one of the promising dispensing technologies to fabricate scaffolds with controllable pore sizes and porosities. There are many factors to affect the printing quality in pneumatic systems. An effective method is required to help users to systematically select proper operational parameters to print strands with desired width. In this paper, the DoE method is introduced to evaluate operational parameters and their interactions. The solution is further verified using regression tests. Based on the proposed solution, a five steps parameters selection method is proposed and verified to select operational parameters for two targeted strand widths.

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