Engine experiments were performed for measurement of regulated, unregulated, and particulate emissions using a 2.2 L transportation compression ignition engine fueled with blends of diesel and biodiesels derived from Jatropha and waste cooking oils. JB20 (20% v/v Jatropha biodiesel blended with 80% v/v diesel), WCOB20 (20% v/v waste cooking oil biodiesel blended with 80% v/v diesel), and baseline mineral diesel were used as test fuels in this study. Experiments were performed at an engine speed of 2000 rpm at five engine loads (0%, 25%, 50%, 75%, and 100% rated load). Regulated emission results exhibited that JB20 and WCOB20 emitted higher hydrocarbons and carbon monoxide (CO) at low engine loads compared to baseline diesel, whereas WCOB20 exhibited relatively higher oxides of nitrogen (NOx) emissions compared to baseline diesel. Unregulated emissions were higher at low engine loads and decreased with increasing engine load. Biodiesel blends showed relatively higher methane and ethylene trace emissions compared to baseline diesel, whereas WCOB20 showed higher formaldehyde, formic acid, iso-pentane, sulfur dioxide, and n-octane emissions compared to diesel at no load. Particulate number concentrations were relatively higher from biodiesel blends compared to baseline diesel at most engine operating conditions.