We have developed a three-dimensional (3D) bioprinting system capable of multimaterial and multiscale deposition to enable the next generation of “bottom-up” tissue engineering. This area of research resides at the interface of engineering and life sciences. As such, it entails the design and implementation of diverse elements: a novel hydrogel-based bioink, a 3D bioprinter, automation software, and mammalian cell culture. Our bioprinter has three components uniquely combined into a comprehensive tool: syringe pumps connected to a selector valve that allow precise application of up to five different materials with varying viscosities and chemistries, a high velocity/high-precision x–y–z stage to accommodate the most rapid speeds allowable by the printed materials, and temperature control of the bioink reservoirs, lines, and printing environment. Our custom-designed bioprinter is able to print multiple materials (or multiple cell types in the same material) concurrently with various feature sizes (100 μm–1 mm wide; 100 μm–1 cm high). One of these materials is a biocompatible, printable bioink that has been used to test for cell survival within the hydrogel following printing. Hand-printed (HP) controls show that our bioprinter does not adversely affect the viability of the printed cells. Here, we report the design and build of the 3D bioprinter, the optimization of the bioink, and the stability and viability of our printed constructs.
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Multimaterial and Multiscale Three-Dimensional Bioprinter
Jennifer Campbell,
Jennifer Campbell
Center for Manufacturing Innovation,
Fraunhofer USA,
Brookline, MA 02446
e-mail: jcampbell@fraunhofer.org
Fraunhofer USA,
Brookline, MA 02446
e-mail: jcampbell@fraunhofer.org
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Ian McGuinness,
Ian McGuinness
Center for Manufacturing Innovation,
Fraunhofer USA,
Brookline, MA 02446
e-mail: imcguinness@fraunhofer.org
Fraunhofer USA,
Brookline, MA 02446
e-mail: imcguinness@fraunhofer.org
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Andre Sharon,
Andre Sharon
Mem. ASME
Center for Manufacturing Innovation,
Fraunhofer USA,
Brookline, MA 02446;
Center for Manufacturing Innovation,
Fraunhofer USA,
Brookline, MA 02446;
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Alexis F. Sauer-Budge
Alexis F. Sauer-Budge
Center for Manufacturing Innovation,
Fraunhofer USA,
Brookline, MA 02446;
Fraunhofer USA,
Brookline, MA 02446;
Biomedical Engineering Department,
Boston University,
Boston, MA 02215
e-mail: asauerbudge@fraunhofer.org
Boston University,
Boston, MA 02215
e-mail: asauerbudge@fraunhofer.org
Search for other works by this author on:
Jennifer Campbell
Center for Manufacturing Innovation,
Fraunhofer USA,
Brookline, MA 02446
e-mail: jcampbell@fraunhofer.org
Fraunhofer USA,
Brookline, MA 02446
e-mail: jcampbell@fraunhofer.org
Ian McGuinness
Center for Manufacturing Innovation,
Fraunhofer USA,
Brookline, MA 02446
e-mail: imcguinness@fraunhofer.org
Fraunhofer USA,
Brookline, MA 02446
e-mail: imcguinness@fraunhofer.org
Holger Wirz
Andre Sharon
Mem. ASME
Center for Manufacturing Innovation,
Fraunhofer USA,
Brookline, MA 02446;
Center for Manufacturing Innovation,
Fraunhofer USA,
Brookline, MA 02446;
Alexis F. Sauer-Budge
Center for Manufacturing Innovation,
Fraunhofer USA,
Brookline, MA 02446;
Fraunhofer USA,
Brookline, MA 02446;
Biomedical Engineering Department,
Boston University,
Boston, MA 02215
e-mail: asauerbudge@fraunhofer.org
Boston University,
Boston, MA 02215
e-mail: asauerbudge@fraunhofer.org
1Corresponding author.
Manuscript received March 20, 2015; final manuscript received August 3, 2015; published online September 29, 2015. Assoc. Editor: Ibrahim Ozbolat.
J. Nanotechnol. Eng. Med. May 2015, 6(2): 021005 (7 pages)
Published Online: September 29, 2015
Article history
Received:
March 20, 2015
Revised:
August 3, 2015
Citation
Campbell, J., McGuinness, I., Wirz, H., Sharon, A., and Sauer-Budge, A. F. (September 29, 2015). "Multimaterial and Multiscale Three-Dimensional Bioprinter." ASME. J. Nanotechnol. Eng. Med. May 2015; 6(2): 021005. https://doi.org/10.1115/1.4031230
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